Composite

Part:BBa_K4409016:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


gRNA (AAG+ GAPDH as guide)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of Cas12 handle with AAG present designed by previous groups is utilized to achieve a high sensitivity for the igRNA and Cas12 enzyme. The guide sequence targeting GAPDH dsDNA is chosen because GAPDH dsDNA is easily obtained in high quantity due to the abundant presence of GAPDH genes in cells and can be easily amplified.


Source

The sequence that we designed is based on BBa_K2961003 for Cas12 handle with AAG present, and BBA_K4409017 for the guide sequence targeting a DNA sequence of GAPDH.

References